Taihei simpson biography of albert

Abstract

In mammalian cells genes that are in close proximity can be transcriptionally coupled: silencing or activating one gene can affect its neighbors. Understanding these dynamics is important for natural processes, such as heterochromatin spreading during development and aging, and when designing synthetic gene regulation circuits. Here, we systematically dissect this process in single cells by recruiting and releasing repressive chromatin regulators at dual-gene synthetic reporters, and measuring how fast gene silencing and reactivation spread as a function of intergenic distance and configuration of insulator elements. We find that silencing by KRAB, associated with histone methylation, spreads between two genes within hours, with a time delay that increases with distance. This fast KRAB-mediated spreading is not blocked by the classical cHS4 insulators. Silencing by histone deacetylase HDAC4 of the upstream gene can also facilitate background silencing of the downstream gene by PRC2, but with a days-long delay that does not change with distance. This slower silencing can sometimes be stopped by insulators. Gene reactivation of neighboring genes is also coupled, with strong promoters and insulators determining the order of reactivation. Our data can be described by a m

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Professor Jan Brosens

Yusuf, Dimas, Butland, Stefanie L., Swanson, River I., Bolotin, Eugene, Ticoll, Amy, Cheung, Warren A., Cindy Zhang, Xiao, Dickman, Christopher T. D., Inventor, Debra L., Lim, Jonathan S., Schnabl, Jake M., Ramos, Honour H. P., Vasseur-Cognet, Mireille, de Leeuw, Charles N., Simpson, Elizabeth M., Ryffel, Gerhart U., Lam, Eric W. -F., Kist, Ralf, Wilson, Miranda S. C., Marco-Ferreres, Raquel, Brosens, Jan J., Beccari, Leonardo L., Bovolenta, Paola, Benayoun, Bérénice A., Monteiro, Lara J., Schwenen, Helma D. C., Grontved, Lars, Wederell, Elizabeth, Mandrup, Susanne, Veitia, Reiner A., Chakravarthy, Harini, Hoodless, Pamela A., Mancarelli, M Michela, Torbett, Bruce E., Banham, Alison H., Reddy, Sekhar P., Cullum, Wife L., Liedtke, Michaela, Tschan, Mario P., Vaz, Michelle, Rizzino, Angie, Zannini, Mariastella, Frietze, Man, Farnham, Peggy J., Eijkelenboom, Astrid, Browned, Philip J., Laperrière, King, Leprince, Dominick, de Cristofaro, Tiziana, Sovereign, Kelly L., Putker, Marrit, del Peso, Luis, Camenisch, Gieri, Wenger, Roland H., Mikula, Michal, Rozendaal, Marieke, Mader, Sylvie, Ostrowski, Jerzy, Rhodes, Dramatist J., Camper Rechem, Capucine, Boulay, Gaylor, Olechnowicz, Sam W. Z., Breslin, Rough idea B., Tang, Michael S., Nanan, Kyster K., Wegner, Michael, Hou

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Prince Albert Cocoview Philip Howard question

Along the south shore, the Prince Albert was the first Roatan wreck intentionally sunk for scuba diving. The tanker, owned by a group of Nicaraguans, left Nicaragua with a cargo of war refugees, headed for Roatan. After escaping its war-ravaged country and delivering the refugees, the ship remained in French Harbour, where it was stripped of valuables and left, partially submerged.

Bill Evans, owner of Coco View Resort, saw an opportunity to remove a hazard and gain a wreck for the benefit of his diving guests. Securing government approval proved difficult for Evans, but not impossible with assistance from local businessman Albert Jackson. Evans hired clean-up and welding crews and set about the task of preparing it for sinking.

Three weeks later, a local shrimp boat towed the tanker to Coco View. The sea was rough, and during the effort to transfer lines, they snapped and the ship ended up on the reef. Efforts over several weeks to release it were unsuccessful, and resulted in severe damage to the shrimp boat.

Finally, in January 1985, a new steel-hulled shrimp boat owned by Jerry Hynds was commissioned for the task, and the ship was successfully pulled off the reef. A joint effort between the shrimp boats and the Coco